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To identify possible sites for mutation, the ABDs of the NET family were analysed to look for residues that were homologous to all members except NET3B, which has been shown not to bind actin. Once potential residues were identified, point mutants were designed and cloned alongside an ABD deleted construct. The clones were then expressed in a dominant negative approach using transient expression techniques.
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I turn back and say, "it Medzmama (grandmother in Armenian), it Medzmama right there!" And point to the bottom where she is. They say no one is there are looking where I am pointing exactly. I turn back to look and she is there. Bannon’s camp says, no, that’s not the case at all. Bannon told NBC, for example, it’s "total nonsense." He "de operationalized" the NSC after Obama’s White House was micromanaging foreign policy with it, and that was the real goal, Bannon said. That’s some heavy spin.
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